Coding

Part:BBa_K4060509:Design

Designed by: Cheng-Ju Lu   Group: iGEM21_NYCU-Taipei   (2021-10-21)


mCherry_QPAS1 fusion protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 709
    Illegal SpeI site found at 745
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 709
    Illegal SpeI site found at 745
    Illegal PstI site found at 352
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 709
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 709
    Illegal SpeI site found at 745
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 709
    Illegal SpeI site found at 745
    Illegal PstI site found at 352
    Illegal NgoMIV site found at 901
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construction of this part is according to igem assembly protocol.


Source

The QPAS1 gene and mCherry gene is from Addgene plasmid pKA-207I10

References